AKTIVITAS ANTIBAKTERI AKTINOMISETES LAUT DARI PULAU ENGGANO

Shanti Ratnakomala, Pamella Apriliana, Fahrurrozi Fahrurrozi, Puspita Lisdiyanti, Wien Kusharyoto
10.14203/beritabiologi.v15i3.2258
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Abstract

Marine actinomycetes were isolated from mangrove sediment from the coast in Enggano, Bengkulu Province, Indonesia using the medium NBRC No. 802 modified by the addition of 2% NaCl. A total of 29 isolates of actinomycetes were isolated from three mangrove sediment samples and evaluated their potential to produce bioactive metabolites. Screening of 29 isolates marine actinomycetes isolates were performed against three bacterial pathogens had been done. Bacteria test used was Escherichia coli NBRC 14 237, Staphylococcus aureus NBRC13 276, and Bacillus subtilis NBRC 3134. Screening result showed that seven isolates have inhibitory effects against bacteria test and 22 other isolates have no inhibition. Of the seven isolates, one isolate has inhibitory effect against the growth of Gram-negative bacteria Escherichia coli, while six other isolates inhibit Gram-positive bacteria Bacillus subtilis and Staphilococcus aureus. It was concluded that, of the 29 isolates conducted in the experiment, seven isolates produce antibacterial compounds on agar medium. Molecular identification of 23 isolates were identified based on  the gene 16S RNA sequences showed that 22 isolates belong to the genus Streptomyces and one strain belongs to the genus Dermacoccus.

Keywords

marine actinomycetes, antibacterial, Enggano Island.

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References

Berdy J. 2012. Thoughts and Facts about Antibiotics: Where We are Now and Where We are Heading. The Journal of Antibiotics 65, 385–395.

Busti E, P Monciardini, L Cavaletti, R Bamonte, A Lazzarini, M Sosio and S Donadio. 2006. Antibiotic Producing Ability by Representatives of a Newly Discovered Lineage of Actinomycetes. Microbiology 152, 675–683.

Dharmaraj S. 2010. Marine Streptomyces as a Novel Source of Bioactive Substances. World Journal of Microbiology and Biotechnology 26, 2123–2139.

Ellaiah P and Reddy APC. 1987. Isolation of Actinomycetes from Marine Sediments of Visakhapatnam, East Coast of India. Indian Journal of Marine Science 16, 34–135.

Fenical W and PR Jensen. 2006. Developing a New Resource for Drug Discovery: Marine Actinomycete Bacteria. Nature Chemical Biology 2, 666–73.

Hamada M, T Iino, T Tamura, T Iwami, S Harayama and K Suzuki. 2009. Serinibacter salmoneus gen. nov, sp. nov., an Actinobacterium Isolated from the Intestinal Tract of Fish, and Emended Descriptions of the Families Beutenbergiaceae and Bogoriellaceae. International Journal of Systematic and Evolutionary Microbiology 59, 2809-2814.

Hodges TW, M Slattery, JB Olson. 2012. Unique Actinomycetes from Marine Caves and Coral Reef Sediments Provide Novel PKS and NRPS Biosynthetic Gene Clusters. Marine Biotechnology 14, 270–280.

Holguin G, P Vazquez, and Y Bashan. 2001. The Role of Sediment Microorganisms in the Productivity, Conservation, and Rehabilitation of Mangrove Ecosystems: An Overview. Biology and Fertility of Soils 33, 265–278.

Jensen PR, TJ Mincer, PG Williams and W Fenical. 2005. Marine Actinomycete Diversity and Natural Product Discovery. Antonie van Leeuwenhoek 87, 43–8.

Lam KS. 2006. Discovery of Novel Metabolites from Marine Actinomycetes. Current Opinion in Microbiology 9, 245–51.

Lane ALand BS Moore. 2011. A Sea of Biosynthesis: Marine Natural Products Meet the Molecular Age. Natural Product Reports 28(2), 411–428.

Manivasagan P, KH Kang, K Sivakumar, Li ECY -Chan, HM Oh and SK Kim. 2014. Marine Actinobacteria: An Important Source of Bioactive Natural Products. Environmental Toxicology and Pharmacology 38,172–188.

Ozcan K, A Uzel, E Bedir. 2015. Anti-Microbial Activity of Chloramphenicol from Streptomyces sp.10CM9. Procedia - Social and Behavioral Sciences 195, 1736 – 1739.

Ramesh S and Mathivanan N. 2009. Screening of Marine Actinomycetes Isolated from the Bay of Bengal, India for Antimicrobial Activity and Industrial Enzymes. World Journal of Microbiology and Biotechnology 25, 2103–11.

Saito H and KI Miura 1963. Preparation of Transforming DNA by Phenol Treatment. Biochimica et Biophysica Acta 72, 619-629.

Sosovele ME, KM Hosea and TJ Lyimo. 2012. In Vitro Antimicrobial Activity of Crude Extracts from Marine Streptomyces Isolated from Mangrove Sediments of Tanzania. Journal of Biochemical Technology 3(4), 431-435.

Stewart EJ. 2012. Growing Unculturable Bacteria. Journal of Bacteriology 194, 4151–4160.

Susilowati DN, RD Hastuti and E Yuniarti. 2007. Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1. 1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407. Jurnal Agro Biogen 3(1), 15-23.

Terahara T, T Kobayashi, C Imada. 2013. An Effective Method Based on Wet-Heat Treatment for the Selective Isolation of Micromonospora from Estuarine Sediments. World Journal of Microbiology and Biotechnology 29, 1677–1684.

Todar K. 2011.Staphylococcus aureus and Staphylococcus Diseas http://www.textbookofbacteriology.net/staph.html. (Diunduh 2 Agustus 2015).

Wahyuni DS, MB Sudarwanti, and P Lisdiyanti. 2014. Screening of Antibacterial Activities of Actinomycetes Isolates from Indonesia. Global Veterinaria 13 (2), 266-272.

Weber T, P Charusanti, EM Musiol-Kroll, X Jiang, Y Tong, HU Kim and SY Lee. 2015. Metabolic Engineering of Antibiotic Factories: New Tools for Antibiotic Production in Actinomycetes. Trends in Biotechnology 33 (1), 15-26.

Zotchev SB. 2012. Marine Actinomycetes as an Emerging Resource for the Drug Development Pipelines. Journal of Biotechnology 158, 168–175.

DOI: https://doi.org/10.14203/beritabiologi.v15i3.2258

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