OPTIMASI PRODUKSI SERTA ANALISIS AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA SENYAWA EKSOPOLISAKARIDA DARI JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA CAIR

Iwan Saskiawan, Misbahul Munir, Suminar S Achmadi
10.14203/beritabiologi.v15i2.2299
| Abstract views: 1723 | PDF views: 1827

Abstract

Pleurotus ostreatus is well known as an oyster mushroom that is very popular because of its high nutritional value and pharmaceutical component. The aim of this research was to study the antioxidant and antibacterial activity of exopolysaccharides from P. ostreatus grown on liquid medium.The P. ostreatus was grown on Mushroom Complete Medium (MCM) containing various types of Carbon (C) and Nitrogen (N) sources, i.e glucose, lactose, amylose and sucrose as a Carbon sources and yeast extract, polypeptone, NH4Cl, and NH4NO3 as a Nitrogen sources. The results showed that sucrose and yeast extract were the best source of Carbon and Nitrogen that produced 208 and 100 mg/L of exopolysaccharides.  The exopolysaccharide exhibited antimicrobial activity against Bacillus subtilis and Escherichiacoli. However, it did not have antimicrobial activity against Saccharomyces cerevisiae.  In addition, the exopolysaccharide indicated to have an antioxidant activity.

Keywords

antimicrobial, antioxidant, exopolysaccharide, oyster mushroom

Full Text:

PDF

References

Chen W, Z Zhao, SF Chen, and YQ Li. 2007. Optimization for the production of exopolysaccharides from Fomes fomen-tarius in submerged culture and its antitumor effect in vitro. Bioreource Technology 99,3187-3194.

Cho YS, JS Kim, DE Crowley, and BG Cho. 2002. Growth promotion of the edible fungus Pleurotus ostreatus by fluorescent pseudomonads. Microbiology Letters 218,271-276.

Cho EJ, J Young, HY Chang, and JW Yun. 2006. Production of exopolysaccharides by submerged mycelial culture of a mushroom Tremella fuciformis. Journal ofBiotechnology 127,129-140.

Cohen R, L Persky, and Y Hadar. 2002. Biotechnological ap-plication and potential of wood-degrading mushrooms of the Genus Pleurotus. Applied Microbiology andBiotech-nology. 58,582-594.

Dewi FK. 2010. Aktivitas antibakteri ekstrak etanol buah mengkudu (Morinda citrifolia, Linnaeus) terhadap bakteri pembusuk daging segar. Surakarta (ID): Universitas Sebelas Maret.[skripsi]

Dubois M, KA Gilles, JK Hamilton, PA Rebers, and F Smith. 1956. Colorimetric method for determination of sugar and related substance. AnalyticalChemistry. 28:350-356.

Handayani R and J Sulistyo. 2008. Sintesis senyawa flavonoid ?-glikosida secara reaksi transglikosilasi enzimatik dan aktivitasnya sebagai antioksidan. Biodiversitas 9,1-4.

Hwang HJ, SE Kim, JW Choi, and JW Yun. 2003. Production and characterization of exopolysaccharides from sub-merged culture of Phellinus linteus KCTC 6190. Enzyme and Microbial Technology 33,309-319.

Jose N and KK Janardhanan. 2000. Antioxidant and anti-tumor activity of Pleurotus florida. CurrenttScience 79,941-943.

Kim HO, JM Lim, JH Joo, SW Kim, HJ Hwang, JW Choi, and JW Yun. 2004. Optimization of submerged cul-ture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea. Biore-sourceTechnology. 96:1175-1182.

Kim SW, HJ Hwang, JP Park, YJ Cho, CH Song, and JW Yun. 2002. Mycelial growth and exobiopolymer pro-duction by submerged culture of various edible mush-rooms under different media. Letters in Applied Microbiology 34,56-61.

Lin ES and SC Sung. 2006. Cultivating conditions influence exopolysaccharides production by the edible Basidiomycete Antrodia cinnamomea in submerged culture. International Journal of Food Microbiology 108,182-187.

Miles PG and ST Chang. 1997. Mushrooms Biology. Concise Basics and Current Developments. Singapore (SG), World Scientific.

Norrel SA and KE Messley. 1997. Microbiology Laboratory Manual Principles and Applications. New Jersey (US), Prentice Hall.

Pelczar MJJ and ECS Chan. 1986. Dasar-Dasar Mikrobiologi. Hadioetomo RS, Imas T, Tjitrosomo SS, Angka SL, penerjemah; Jakarta (ID): UI-Pr. Terjemahan dari: Ele-ment of Microbiology.

Pokhrel CP and S Ohga. 2007. Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes. Food Chemistry 105,641-646.

Pushpa H and KB Purushothama. 2010. Antimicrobial activity of Lyophyllumdecates an edible wild mushroom. World Journal of Agriculture Science 6(5),506-509.

DOI: https://doi.org/10.14203/beritabiologi.v15i2.2299

Refbacks

  • There are currently no refbacks.