Evi Triana
| Abstract views: 2762 | PDF views: 1891


Several Escherichia coli strains are pathogenic. Excessive and noncompliant use of antibiotics and disinfectants may cause bacteria to build resistance mechanisms. Forming biofilms cause eradicatation more difficult. An effective cleaning action required antibiofilm and antimicrobial agents that have different mechanisms with antibiotics and disinfectants. Bacteriophages are potential candidates because they meet these requirements. Bacteriophages produce specific polysaccharide lyase enzymes capable of degrading biofilm extracellular polymeric matrix. Study was aimed to determine concentrations of specific bacteriophage showing Escherichia coli antibiofilm activity was conducted. The results of this study showed that the most effective concentrations bacteriophage EC RTH 04 to prevent, inhibit, and degrade Escherichia coli EC 3 biofilms were 106, 102, dan 102 respectively.


biofilm, antibiofilm, Escherichia coli, bacteriophage

Full Text:



Adams, M.H., 1959. Bacteriophages. InterScience Publishers, Inc. New York.

Azeredo, J. and Sutherland, W., 2008. The use of phages for the removal of infectious biofilms. Current Biotechnology, 9, pp. 261 – 266.

Azizian, R., Nasab, S.D.M. and Ahmadi, N.A., 2013. Bacteriophages as a novel antibacterial agent in industry and medicine. Journal of Paramedical Science, 4(4), pp. 93 – 101.

Beloin, C., Roux, A. and Ghigo, J.M., 2008. Escherichia coli biofilms. Current Topics of Microbiology and Immunology, 322, pp. 249 – 289.

Bentley, R. and Meganathan, R., 1982. Biosynthesis of vitamin K (menaquinone) in bacteria. Microbiological Reviews, 46(3), pp. 241 – 280.

Chan, B.K. and Abedon, S.T., 2015. Bacteriophage and their enzyme in biofilm control. Current Pharmaceutical Design, 21, pp. 85 – 99.

Costerton, J.W., Lewandowski, Z., Caldwell, D.E., Korber, D.R., and Lappin-Scott, H.M., 1995. Microbial Biofilms. Dalam: Annual Review of Microbiology. Vol. 49. Ornston, L.N., Ballows, A., and Greenberg, E.P. (eds) Annu Rev Inc. Palo Alto.

Donlan, R.M., 2002. Biofilms: Microbial life on surfaces. Emerging Infectious Disease, 8(9), pp. 881 – 890.

Esmat, M.M., Abdelhamid, A.G., Esmael, A., Nasr-Eldin, M.A., Abolmaaty, S., Hassan, M.G. and Khattab, A.A., 2018. Antibiotics and phage sensitivity as intervention and controlling Escherichia coli isolated from clinical speciments. Journal of Pure and Applied Microbiology. doi://DX.DOI.ORG/10.22207/JPAM11.4.12

Forbes, B.A., Sahm, D.F. and Weissfeld, A.S., 2007. Bailey & Scott’s Diagnostic Microbiology. 12th ed. Mosby Elsevier. St. Louis.

Harper, D.R., Parracho, H.M.R.T. and Walker, J., 2014. Bacteriophages and biofilms. Antibiotics, 3, 270-284.

Hudault, S., Guignot, J., and Servin, A.L., 2001. Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection. Gut, 49(1), pp. 47 – 55. doi:10.1136/gut.49.1.47.

Labrie, S.J., Samson, J.E. and Moineau, S., 2010. Bacteriophage resistance mechanisms. National Review of Microbiology, 8(5), pp. 317 – 327.

O’Toole, G.A., Kaplan, H.B., and Kolter, R., 2000. Biofilm formation as microbial development. Annual Review of Microbiology, 54, pp. 49 – 79.

Parisien, A., Allain, B., Zhang, J., Mandevillen, R. and Lan, C.G., 2008. Novel alternatif to antiobiotics: bacteriophages, bakterial cell wall hidrolases and antimicrobial peptides. Journal of Applied Microbiology, 104, pp. 1 – 13.

Peterson, S.B., Irie, Y., Borlee, B.R., Murakami, K., Harrison, J.J., Colvin, K.M. and Parsek, M.R., 2011. Different Methods for Culturing Biofilms In Vitro. Dalam: Biofilm Infections. Bjarnsholt, T., Jensen, P.O., and Christopersen, L. (eds.) pp. 251 – 266. Springer, New York.

Reisner, A., Maierl, M., Jorger, M., Krause, R., Berger, D., Haid, A., Tesic, D. and Zechnerc, E.L., 2014. Type 1 fimbriae contribute to catheter-associated urinary tract infections caused by Escherichia coli. Journal of Bacteriology, 196, pp. 931 – 939.

Khan, M.S.A., Ahmad, I., Sajid, M. and Cameotra, S.S., 2014. Current and Emergent Control Strategies for Medical Biofilms. Dalam: Antibiofilm Agents: From Diagnosis to Treatment and Prevention. Rumbaugh, K.P. and Ahmad, I. (eds.) pp. 117 – 159. Springer-Verlag, Berlin.

Ryan, K.J., 2004a. Normal Microbial Flora. Dalam: Sherris Medical Microbiology: An introduction to infectious disease. 4th ed. Ryan, K.J. and Ray, C.G. (eds.) pp. 141 –148. McGraw Hill, New York.

Ryan, K.J., 2004b. Enterobacteriaceae. Dalam: Sherris Medical Microbiology: An introduction to infectious disease. 4th ed. Ryan, K.J. and Ray, C.G. (eds.) pp. 343 – 372. McGraw Hill, New York.

Ryan, K.J., 2004c. Antimicrobial Resistance. Dalam: Sherris Medical Microbiology: An introduction to infectious disease. 4th ed. Ryan, K.J. and Ray, C.G. (eds.) pp. 215 – 228. McGraw Hill, New York.

Simoes, M., Simoes, L.C. and Viera, M.J., 2010. A Review of current and emergent biofilm control strategies. Food Science and Technology, 43, pp. 573 – 583.

Soto, S.M., Marco, F., Guiral, E. and Vila, J., 2011. Biofilm Formation in Uropathogenic Escherichia coli Strains: Relationship with Urovirulence Factors and Antimicrobial Resistance. Dalam: Clinical Management of Complicated Urinary Tract Infection. Nikibakhsh, A.A. (ed.) In Tech, Rijeka, Croatia.

Sulakvelidze, A. and Kutter, E., 2005. Bacteriophages Therapy in Humans. Dalam: Bacteriophages Biology and Application. Kutter, E. and Sulakvelidze, A. (eds.) CRC Press, Boca Raton.

Sutherland, I.W., Hughes, K.A., Skillman, L.C. and Trait, K., 2004. The interaction of phage and biofilms. FEMS Microbiology Letter, 232, pp. 1 – 6.

Tortora, G.J., Funke, B.R. and Case, C.L., 2001. Microbiology: An Introduction. 7th ed. Wesley Longman, Inc., New York.

Zuroff, T.R., Bernstein, H., Randolfi, J.L., Taracido, L.J., Stewart, P.S. and Carlson, R.P., 2010. Robustness analysis of culturing perturbations on Escherichia coli colony biofilm beta-lactam and aminoglycoside antibiotic tolerance. BMC Microbiology, 10, pp. 185.


  • There are currently no refbacks.