Kajian Pendahuluan: Perpindahan Gen dari Tanaman Kentang Transgenik Katahdin RB ke Tanaman Kentang Non Transgenik

Abstract

ABSTRACT
Preliminary study: Gene transfer from transgenic potato Katahdin RB to non transgenic
potato. One of the concerns associated with the release of transgenic crops, is the possibility
of the gene flow from transgenic crops to neighboring crops of the same species or to related
species. In plants, gene flow is a routine process occur through the natural hybridization. The
opportunity for gene flow occur depends principally on two factors, the degree of sexual
compatibility between donor and recipient species, and the physical distance between the
two. The experiment was conducted to determine whether the gene flow from transgenic
potato Katahdin RB to non transgenic was occurred, based on selection using a 50 mg/l
kanamycin, and to estimate gene flow mediated by natural hybridization at different isolation
distances. Preliminary result indicated that a rapid and simple method using MS0 liquid media
with kanamycin 50 mg/l was effective for screening the seeds. There was a gene flow from
transgenic potato Katahdin RB to non transgenic, based on a rapid and simple selection
method using 50 mg/l of kanamycin as selectable marker. The isolation distance used in the
study were 0.8, 1.6, 2.4, 3.2, 4.0, 4.8, 5.6, 6.4, 7.2, 8.0, 8.8, 9.6, 10.4, and 11.2 m from the row of
transgenic potato Katahdin RB. The gene flow through natural hybridization at a isolation
distances of (0.8 - 1.6 m), (2.4 – 4 m), and (4.8 – 6.4 m) from transgenic to non transgenic plants
were 13.78, 10.92, and 3.82%, respectively. At a distance of 7.2 – 8 m, the frequency of gene flow
was declined to 0%. The frequency of gene flow from transgenic potatoes to non transgenic
potatoes markedly decreased by increasing the isolation distance, and was negligible at 7.2 m.
Key words : natural hybridization, transgenic potato RB, kanamycin selection