Isolasi Bakteri Pendegradasi Phenanthrene dari Batanta-Salawati Raja Ampat Papua

Abstract

ABSTRACT
Isolation of Bacteria Degrading Phenanthrene in Batanta- Salawati Districts Raja Ampat
Papua. Polycyclic aromatic hydrocarbons (PAHs) are important environmental contaminants
in soil and water. These compounds have a potential risk to human health, as many of them are
carsinogenic and toxic to marine organisms such as diatome, gasthrophode, mussel, and fish.
Phenanthrene is one of the hazardous hydrocarbon compounds. The purpose of this research
was to characterize microbial strains from Batanta-Salawati Raja Ampat Papua Island and their
ability to remove phenanthrene. Two isolates were identified at their physiological characteristics
based on salinity tolerance, pH tolerance and the composition of nitrogen base. Molecular
identification based on 16S rRNA gene sequences indicated that bacteria had the highest
similarity with Rhodobacteraceae bacterium F9 and Roseobacter sp. RW 37.
Rhodobacteraceae bacterium F9 could grow optimum on ONR7a media with 5% salinity and
at pH of 5-7,5 while Roseobacter sp. RW 37 could grow optimum on ONR7a media with 2%
salinity and at pH of 6,2-7,5.
Key words: Phenanthrene, physiological characteristic, molecular identification, Raja Ampat