Toksisitas Isolat Bacillus thuringiensis yang Mengandung Gen cry 1A Terhadap Hama Penggerek Batang Jagung, Ostrinia furnacalis Guenee

Abstract

ABSTRACT
Cry genes isolated from Bacillus thuringiensis produce crystal proteins that exhibit a high
insecticidal activity against several plant pests. The objectives of this experiment were to
detect the presence of cry1A sequences from several local Bacillus thuringiensis isolates
multiplied by Lep1A-Lep1B and Lep2A-Lep2B primers using PCR technique and to determine
their toxicity against Ostrinia furnacalis, maize stemborer. From 59 tested isolates, 6 of them
gave PCR products, two DNA bands, first was 490 bp, the expected size of Lep1A-Lep1B
primers, and second was 986 bp, the expected size of Lep2A-Lep2B primers. These isolates
were Jtg 2151, 243, Cib 551, Lam 752, Lam 762 and C 522. All of tested isolates showed
potentially high toxicity against maize stemborer.
Key words: B. thuringiensis, cry1A, Ostrinia furnacalis, PCR.
Kata kunci: B. thuringiensis, cry1A, Ostrinia furnacalis, PCR.