Karakterisasi Enzim Nitril Hidratase dan Amidase dari Pseudomonas sp. BP3 dalam Biokonversi Adiponitril menjadi Asam Adipat

Abstract

ABSTRACT
Characterization of Nitrile Hydratase and Amidase of Pseudomonas sp BP3 in Bioconversion
of Adiponitrile to Adipic Acid. Adipic acid is a commercially important compound, primarily
used as precursor for the production of nylon 6.6. It is also used for plasticizer, fibers, and food
additive. Synthesis of adipic acid by chemical means requires large amount of energy and
concentrated acid. It also produces N2O as by product, which is very toxic and suspected
causes depletion of the ozone layer. The purpose of this research was to study the
bioconversion of adiponitrile by Pseudomonas sp. BP3 and to characterize the involved enzymes
in the whole cell. Pseudomonas sp. BP3 was able to utilize adiponitrile as the sole source of
carbon and nitrogen. It’s doubling time (td) and growth rate constant (?) during the growth in
adiponitrile were 2 hours and 0.346/h, respectively. The optimum pH and temperature of nitrilehydratase
were pH 7.0 and 30°C, respectively, while those of amidase were pH 6 and 50°C.
Vmax and Ks of nitrile hydratase were 8.3 nM/ml.min. and 55.56 mM, respectively, and of
amidase were 5,9 nM/ml.min and 50 mM. The rate of adiponitrile consumption was 0.245 mM/
h and of adipic acid formation was 0.181 mM/h. The yield of bioconversion of adiponitrile and
adipamide were about 50 % and 25%, respectively.
Key words: Bioconversion, adiponitrile, adipic acid, Pseudomonas sp. BP3, nitrile hydratase,
amidase