CERCOSPORA BRUNFELSIICOLA (FUNGI, MYCOSPHAERELLACEAE), A NEW TROPICAL CERCOSPOROID FUNGUS ON BRUNFELSIA UNIFLORA
Iman Hidayat, Jamjan Meeboon
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Abstract
Cercospora brunfelsiicola on Brunfelsia uniflora is proposed as a new species based on a combination of molecular phylogenetic and morphological data analyses. The molecular phylogenetic analysis based on combined multilocus analyses of the Internal Transcribed Spacer (ITS), part of the elongation factor 1–a gene (EF1–a), and part of the calmodulin (CAL) gene regions showed that C. brunfelsiicola is phylogenetically distinguishable from other Cercospora species, including members of the C. apii s. lat. complex. Morphologically, C. brunfelsiicola differs from other closely related Cercospora species, in particular C. acaciae-mangii, by forming lesions with indistinct margin, larger stromata [(32) 48.5 ± 10.6 (68) ?m diam.], and filiform to narrowly obclavate conidia [(45) 59 ± 9.1 (72) × (2.5) 2.5 ± 0.2 (3) ?m].
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BRAUN, U., NAKASHIMA, C. & CROUS, P. W. 2013. Cercosporoid fungi (Mycosphaerellaceae) 1. Spercies on other fungi, Pteridophyta and Gymnospermae. IMA Fungus 4: 265–345.
CARBONE, I. & KOHN, L. M. 1999. A method for designing primer sets for speciation studies in filamentous Ascomycetes. Mycologia 9: 553–556. http://dx.doi.org/10.2307/3761358
CHOI, Y. W., HYDE, K. D. & HO, W. H. 1999. Single spore isolation of fungi. Fungal Diversity 3: 29–38.
CHUPP, C. 1954. A monograph of the fungus genus Cercospora. Ithaca, New York.
CROUS, P. W. & BRAUN, U. 2003. Mycosphaerella and its anamorphs, 1. Names published in Cercospora and Passalora. CBS Biodiversity Series 1. Utrecht, Netherland: Pp. vii + 571 hlm.
CROUS, P. W., APTROOT, A., KANG, J. C., BRAUN, U. & WINGFIELD, M. J. 2000. The genus Mycosphaerella and its anamorphs. Studies in Mycology 45: 107–121.
CROUS, P. W., GROENEWALD, J. Z., PONGPANICH, K., HIMAMAN, W., ARZANLOU, M. & WINGFIELD, M. J. 2004. Cryptic speciation and host specificity among Mycosphaerella spp. occurring on Australian Acacia species grown as exotics in the tropics. Studies in Mycology 50: 457–469.
EDGAR, R. C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acid Research 32: 1792–1797. http://dx.doi.org/10.1093/nar/gkh340
FARRIS, J. S., KALLERSJÖ, M., KLUGE, A. G. & BULT. C. 1995. Constructing a significance test for incongruence. Syst. Biol. 44: 570–572.
FELSENSTEIN, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.
GROENEWALD, M., GROENEWALD, J. Z. & CROUS, P. W. 2005. Distinct species exist within the Cercospora apii morphotype. Phytophatology 95: 951–959.
GROENEWALD, J. Z., NAKASHIMA, C., NISHIKAWA, J., SHIN, H. D., PARK, J. H., JAMA, A. N., GROENEWALD, M., BRAUN, U. & CROUS, P. W. 2013. Species concepts in Cercospora: spotting the weeds among the roses. Studies in Mycology 75: 115–170. http://dx.doi.org/10.3114/sim0012
KISHINO, H. & HASEGAWA, M. 1989. Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. Journal of Molecular Evolution 29: 170–179.
MEEBOON, J., HIDAYAT, I., NAKASHIMA, C. & TO–ANUN, C. 2007a. Cercospora habenariicola sp. nov. and some new records of cercosporoid fungi from Thailand. Mycotaxon 99: 117–121.
MEEBOON, J., HIDAYAT, I. & TO–ANUN, C. 2007b. Cercosporoid fungi from Thailand 3. Two new species of Passalora and six new records of Cercospora species. Mycotaxon 102: 139–145.
MEEBOON, J., HIDAYAT, I. & TO–ANUN, C. 2007c. An annotated list of cercosporoid fungi in Northern Thailand. Journal of Agricultural Technology 3: 51–63.
NAKASHIMA, C., OETARI, A., KANTI, A., SARASWATI, R., WIDYASTUTI, Y. & ANDO, K. 2010. New species and newly recorded species of Cercospora and allied genera from Indonesia. Mycosphere 1: 315–323.
PHENGSINTHAM, P., CHUKEATIROTE, E., MCKENZIE, E. H. C., MOSLEM, M. A., HYDE, K. D. & BRAUN, U. 2012. Fourteen new records of cercosporoids from Thailand. Maejo International Journal of Science and Technology 6: 47–61.
PHENGSINTHAM, P., BRAUN, U., MCKENZIE, E. H. C., CHUKEATIROTE, E., CAI, L. & HYDE, K. D. 2013. Monograph of cercosporoid fungi from Thailand. Plant Pathology and Quarantine 3: 67–138.
PLOWMAN, T. 1977. Brunfelsia in ethnomedicine. Botanical Museum leaflets 25: 289–320.
STÖVER, B. C. & MÜLLER, K. F. 2010 – TreeGraph 2: Combining and visualizing evidence from different phylogenetic analyses. BMC Bioinformatics 11: 7. http://dx.doi.org/10.1186/1471-2105-11-7
SWOFFORD, D. L. 2002. PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates, Sunderland, Massachusetts.
TAMURA, K., STECHER, G., PETERSON, D., FILIPSKI, A. & KUMAR, S. 2013 – MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30: 2725–2729. http://dx.doi.org/10.1093/molbev/mst197
TO–ANUN, C., HIDAYAT, I. & MEEBOON, J. 2011. Genus Cercospora in Thailand: Taxonomy and Phylogeny (with a dichotomous key to species). Plant Pathology & Quarantine 1: 11–87.
WHITE, T. J., BRUNS, T., LEE, S. & TAYLOR, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. 315–322 pp. In: INNIS, M. A., et al. (Eds.), PCR Protocols: a guide to methods and applications. San Diego, Academic Press.
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