The application of leaf anatomy identifying species of Orchidantha N.E.Br. (Lowiaceae Ridl.) in Malaysia
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Abstract
YUSOFF, N. S. S. M., KALU, M., & SIAM, N. A. 2025. The application of leaf anatomy identifying species of Orchidantha N.E.Br. (Lowiaceae Ridl.) in Malaysia. Reinwardtia 24(1): 17‒25. — Lowiaceae only consists of one genus, Orchidantha, which with thirty-four species has the lowest number of species among the eight families within order Zingiberales. Recently, Orchidantha has garnered attention for its potential medicinal value in treating fever, cough, chest, and back pain. The species are often only found in a small area. The morphology of the flower of Orchidantha is essential to identification but often the flowers are rarely found. As a result, this study aims to investigate vegetative anatomical characteristics of the petiole, midrib, and lamina, which could be used for species identification independent of availability of flowers; by identifying shapes and special characteristic of labellum, stigma, and viscidium. The results of this study shown a marked difference in shape and depth of the petiole grooves, petiole margin, and the number of vascular systems. In conclusion, the study demonstrates that the anatomical‒petiole grooves, petiole margin and number of vascular systems, are useful for identifying species.
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References
ANDERSSON, L. 1998. Marantaceae. In: (KUBITZKI, K. (Eds.)) Flowering Plants· Monocotyledons: Alismatanae and Commelinanae (except Gramineae). Berlin, Heidelberg: Springer Berlin Heidelberg. Pp. 278–293.
DANH, N. D., THUY, D. T. T., LUYEN, N. D., & TUYEN, T. T. 2022. Essential oil from the aerial part of Orchidantha vietnamica K.Larsen and its antimicrobial activity. Vietnam Journal of Science and Technology 60(1): 43–48.
ENNOS, A. R., SPATZ, H. C. & SPECK, T. 2000. The functional morphology of the petioles of the banana, Musa textilis. Journal of Experimental Botany 51(353): 2085–2093.
HOLTTUM, R. E. 1970. The genus Orchidantha (Lowiaceae). The Gardens' Bulletin Singapore 25: 239–247.
LEITE, F. A., DE SOUZA, R. R., DA SILVA SOUZA, G. C., DE LIMA, L. G., FERREIRA, C. P. & BECKMANN-CAVALCANTE, M. Z. 2023. Leaf anatomy and morpho-physiological mechanisms of acclimation to high irradiance in heliconia genotypes. Ciencia e Agrotecnologia 47: Art. E000123.
LEONG-ŠKORNIČKOVÁ, J., TILLICH, H. & NGUYEN, Q. B. 2014. Two new species and one new variety of Aspidistra (Asparagaceae : Nolinoideae) from Southern Vietnam. Gardens' Bulletin Singapore 66(1): 27–37.
LEONG-ŠKORNIČKOVÁ, J., LAMB, A., LIN-TON, J. & GOKUSING, L. 2021. Six new Orchidantha species (Lowiaceae) from Borneo. Gardens’ Bulletin Singapore 73(1): 179–202.
ONG, H. C., LINA, E. & MILOW, P. 2012. Traditional knowledge and usage of medicinal plants among the Semai Orang Asli at Kampung Batu 16, Tapah, Perak, Malaysia. Studies in Ethno- Medicine 6(3): 207–211.
PASINI, D. & MIRJALILI, V. 2006. The optimized shape of a leaf petiole. WIT Transactions on Ecology and the Environment 87: 35–45.
PAUL, V., SHARMA, L., PANDEY, R. & MEENA, R. C. 2017. Measurements of Stomatal Density and Stomatal Index on Leaf/Plant Surfaces. Manual of ICAR Sponsored Training Programme for Technical Staff of ICAR Institutes on―Physiological Techniques to Analyze the Impact of Climate Change on Crop Plants. Pp. 27.
RIYAS, C. T. & MATHEW, D. 2018. Comparative macroscopic and microscopic foliar characterization of twelve species belong to four genera in family Costaceae. International Journal of Research and Analytical Reviews 5(4): 753–758.
ROYER, D. L. 2001. Stomatal density and stomatal index as indicators of paleoatmospheric CO2 concentration. Review of Palaeobotany and Palynology 114: 1–2.
SIMÃO, D. G. & SCATENA, V. L. 2001. (Zingiberales : Heliconiaceae) from the Atlantic forest of southeastern Brazil. Revista Brasileira de Botânica 1: 415–424.
SULTANA, N., AKHI, S. S., HASSAN, M. A. & RAHMAN, M. O. 2019. Morphological and anatomical investigation among six variants of Canna indica L. Bangladesh Journal of Plant Taxonomy 26(2): 219–230.
SUMARDI, I. & WULANDARI, M. 2010. Anatomy and morphology character of five Indonesian banana cultivars (Musa spp.) of different ploidy level. Biodiversitas Journal of Biological Diversity 11(4): 167–175.
SUNANDAR, A. & KAHAR, A. P. 2017. Morphology and anatomy characteristic of pisang awak (Musa paradisiaca cv. awak) in West Kalimantan. Biosaintifika: Journal of Biology & Biology Education 9(3): 579–584.
SYAUQINA, M. Y., KALU, M. & TAWAN, C. S. & SYAZANA, S. A. 2016. Orchidantha ranchanensis, a new species of Lowiaceae from Sarawak Malaysia. Folia Malaysiana 17(2): 35–42.
TOMLINSON, P. B. 1956. Studies in the systematic anatomy of the Zingiberaceae. Journal of the Linnean Society of London, Botany 55(361): 547–592.
TOMLINSON, P. B. 1961. Morphological and anatomical characteristics of the Marantaceae. Journal of the Linnean Society of London Botany 58(370): 55–78.
ZAHARA, M. 2020. Identification of morphological and stomatal characteristics of Zingiberaceae as medicinal plants in Banda Aceh, Indonesia. IOP Conference Series: Earth and Environ-mental Science 425: Art. 012046