EXPLORING SPONGE-INHABITING BARNACLES OF EASTERN INDONESIA USING MICRO-CT SCANNING

Pipit Pitriana, Andreas Wessel, Tina Aschenbach, Kristina von Rintelen
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Abstract

We present a morphological study of Indonesian sponge-inhabiting barnacles using standard light microscopy in combination with micro-CT scanning and computer-aided 3D-reconstruction of the external shell morphology. A taxonomic analysis of the material detected four different genera of sponges inhabited by five different species of balanomorph barnacles, two of which are undescribed. Together with conventional morphological examination by dissection, we provide modern non-destructive imaging methods, using micro-CT scanning to enhance our knowledge of the morphological characters of sponge-inhabiting barnacles from eastern Indonesia. Although there were some methodological limitations regarding the contrast-enhancing technique, this study demonstrates micro-CT as a useful non-destructive technique of integrative taxonomy, for the examination of sponge-inhabiting barnacles.

 

Keywords

cirripedia, Indonesian biodiversity, integrative taxonomy, micro-computer tomography, shell morphology

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References

Anderson, D.T. 1994. Barnacles: Structure, function, development and evolution. Chapman & Hall, London, 357 pp.

Broch, H. 1931–1932. Indo-Malayan Cirripedia. Papers from Dr Th. Mortensen’s Pacific Expedition 1914–1916. Videnskabelige Meddelelser fra Dansk Naturhistorisk Forening (Copenhagen), 91: 1–146.

Buytaert, J., Goyens, J., De Greef, D., Aerts, P., & Dirckx, J. 2014. Volume shrinkage of bone, brain and muscle tissue in sample preparation for micro-CT and light sheet fluorescence microscopy (LSFM). Microscopy and Microanalysis, 20 (4): 1208–1217. https://doi.org/10.1017/S1431927614001329

Darwin, C. 1854. A monograph on the subclass Cirripedia with figures of all the species. The Balanidae, the Verrucidae, etc. Ray Society, London, 684 pp, pls. 30. (1854 published 1855).

Faulwetter, S., Dailianis, T., Vasileiadou, A. & Arvanitidis, C. 2013. Contrast enhancing techniques for the application of micro-CT in marine biodiversity studies. Microscopy and Analysis, 27 (2): S4-S7.

Foster, B.A. 1978. The Marine Fauna of New Zealand: Barnacles (Cirripedia: Thoracica). New Zealand Oceanographic Institute Memoir, 69: 1–160.

Friedrich, F. & Beutel, R. G. 2008. Micro-computer tomography and a renaissance of insect morphology. Paper presented at the Proceeding SPIE 7078, Developments in X-Ray Tomography VI, San Diego, California.

Gignac, P.M., Kley, N.J. & Clarke, J. A. 2016. Diffusible iodinebased contrast-enhanced computed tomography (diceCT): an emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues. Journal of Anatomy, 228 (6): 889–909.

Gili, J.M. & Coma, R. 1998. Benthic suspension feeders: their paramount role in littoral marine food webs. Trends in Ecology and Evolution, 13: 316-321.

Heimel, P., Swiadek, N.V., Slezak, P., Kerbl, M., Schneider, C., Nurnberger, S., Redl, H., Teuschl, A.H. & Hercher, D. 2019. Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration. Contrast Media & Molecular Imaging, 2019: 15. https://doi.org/10.1155/2019/7483745

Hoek, P.P.C. 1913. The Cirripedia of the Siboga Expedition. B. Cirripedia Sessilia. Siboga Ex-peditie Monographe XXXIb. E.J. Brill, Leiden, 129–275. https://doi.org/10.5962/bhl.ti-tle.12959

Hosie, A.M., Fromont, J., Munyard, K., Jones, D.S. 2019. Description of a new species of Membranobalanus (Crustacea, Cirripedia) from southern Australia. ZooKeys 873: 25-42. https://doi.org/10.3897/zookeys.873.35421

Ilan, M., Loya, Y., Brickner, I., and Kolbasov, G.A. 1999. Sponge-inhabiting barnacles on Red Sea coral reefs. Marine Biology 133: 709–716. https://doi.org/10.1007/s002270050512

Kolbasov, G.A. 1993. Revision of the genus Acasta Leach (Cirripedia: Balanoidea). Zoological Journal of the Linnean Society, 109: 395–427. https://doi.org/10.1111/j.1096-3642.1993. tb00307.x

Krüger, P. 1911. Beiträge zur Cirripedienfauna Ostasiens. In: Doflein F (ed.) Beiträge zur Naturgeschichte Ostasiens. Kongelige Bayerische Akademie der Wissenschaften, Munich Mathematische-physikalische Klasse, Abhandlungen Supplement-Band 2(6): 1–72.

Lamarck, J.B.P.A. 1818. Histoire naturelle des animaux sans vertèbres. Tome 5. Deterville, Paris. 612 pp.

Lewis, J.B. 1992. Recruitment, growth and mortality of a coral-inhabiting barnacle Megabalanus stultus (Darwin) upon the hydrocoral Millepora complanata Lamarck. Journal Experiment Marine Biology Ecology 162: 51–64.

Metscher, B. D. 2009a. MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiology, 9: 11.

Metscher, B. D. 2009b. MicroCT for developmental biology: a versatile tool for high-contrast 3D imaging at histological resolutions. Developmental Dynamics (American Association of Anatomists), 238: 632–640. https://doi.org/10.1002/dvdy.21857

Nagler, C., Haug, J.T., Glenner, H. & Buckeridge, J. 2017. Litholepas klausreschi gen. et sp. nov., a new neolepadine barnacle (Cirripedia, Thoracica) on a sponge from the Upper Jurassic lithographic limestones of southern Germany. Neues Jahrbuch für Geologie und Paläontologie Abhandlungen, 284(1): 29–42. http//doi.org//: 10.1127/njgpa/2017/0648.

Noever, C., Keiler, J. & Glenner, H. 2015. First 3D reconstruction of the rhizocephalan root system using MicroCT. Journal of Sea Research: 1–7. http://dx.doi.org/10.1016/j.seares.2015.08.002

Pitriana, P., Valente, L., von Rintelen, T., Jones, D.S., Prabowo, R.E. & von Rintelen, K. 2020. An annotated checklist and integrative biodiversity discovery of barnacles (Crustacea, Cirripedia) from the Moluccas, East Indonesia. ZooKeys, 945: 17–83. https://doi.org/10.3897/zookeys.945.39044.

Rosell, N.C. 1991. Crustacea Cirripedia Thoracica: MUSORSTOM 3 Philippines collection. In: Crosnier A (ed.) Résultats des Campagnes MUSORSTOM, Volume 9. Mémoires du Muséum national d’Histoire naturelle (A) 152: 9–61.

Semple, T.L., Peakall, R. & Tatarnic, N.J. 2019. A comprehensive and user‐friendly framework for 3D data visualisation in invertebrates and other organisms. Journal of Morphology, 280(2): 223–231. https://doi.org/10.1002/jmor.20938

Sulistiono, S., Kawaroe, M., Madduppa, H, & Prabowo, R.E. 2014. Karakteristik morfologi teritip spons Indonesia. Depik, 3(2): 178–186. https://doi.org/10.13170/depik.3.2.1553

Van Syoc, R.J. 1988. Description of Membranobalanus robinae, a new species of sponge barnacle (Cirripedia, Archaeobalanidae) from Baja California, with a key to the genus. Proceedings of the Biological Society of Washington 101: 832–837.

https://www.jstor.org/stable/20106147

Van Syoc, R.J. 2019. Thecostraca: Subclass Sessilia. In Rogers DC & Thorp JH (Eds.), Thorp and Covich's Freshwater Invertebrates: Keys to Palaearctic Fauna, Fourth Edition, pp. 782-787. London: Elsevier.

Van Syoc, R.J. & Winther, R. 1999. Sponge-inhabiting barnacles of the Americas: a new species of Acasta (Cirripedia, Archaeobalanidae), first record from the Eastern Pacific, including discussion of the evolution of cirral morphology. Crustaceana, 72: 467–486. https://doi. org/10.1163/156854099503528.

Van Syoc, R.J., Van Soest, R.W., Xavier, J.R. & Hooper, J.N. 2015. A phylogenetic overview of sponge-inhabiting barnacles and their host specificity (Crustacea, Cirripedia). Proceedings of the California Academy of Sciences (Series 4), 62: 331–357.

Wibowo, R.A., Prabowo, R.E. & Nuryanto, A. 2011. Biodiversitas teritip yang hidup pada spons di Perairan Pantai Kepulauan Karimunjawa. In: Prosiding Kongres dan Seminar Masyarakat Taksonomi Kelautan Indonesia, Jakarta 20–22 September 2011, 219–235.

Yu, M.C., Chan, B.K.K., Achituv, Y. & Kolbasov, G.A. 2017a. Four new sponge-inhabiting barnacles of the genus Acasta (Thoracica: Archaeobalanidae: Acastinae) from the Indo-Pacific. Raffles Buletin of Zoology, 65: 585–615.

Yu, M.C., Kolbasov, G.A., Hosie, A.M., Lee, T-M. & Chan, B.K.K. 2017b. Descriptions of four new sponge-inhabiting barnacles (Thoracica: Archaeobalanidae: Acastinae). Zootaxa, 4277 (2): 151–198. https://doi.org/10.11646/zootaxa.4277.2.1.

Yu, M.C., Kolbasov, G.A., Høeg, J.T. & Chan, B.K.K. 2019. Crustacean-sponge symbiosis: collecting and maintaining sponge-inhabiting barnacles (Cirripedia: Thoracica: Acastinae) for studies on host specificity and larval biology. Journal Crustacean Biology, 39: 522–532. https://doi.org//10.1093/jcbiol/ruz025.

Yu, M.C., Dreyer, N., Kolbasov, G.A., Høeg, J.T. & Chan, B.K.K. 2020. Sponge symbiosis is facilitated by adaptive evolution of larval sensory and attachment structures in barnacles. Proceedings Royal Society B, 287: 20200300. http://dx.doi.org/10.1098/rspb.2020.0300.

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