Muhammad Fakhri Fauzan, Achmad Farajallah, Amir Hamidy
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Leptobrachium from Southeast Asian populations previously were recognized as a single species
Leptobrachium hasseltii Tschudi. Taxonomic reassessment revealed that Leptobrachium consists of some
cryptic species due to similarities in their morphological appearance but diverse in molecular characters.
Among the Indonesian populations, Leptobrachium was split into seven species (L. hasseltii, L. nigrops,
L. waysepuntiense, L. hendricksoni, L. ingeri, L. abbotti, and L. montanum). Specifically, L. hasseltii is
separated into two groups; the Sumatran-Javan and the Bali populations. In this study, we analyzed the
morphological data of 100 individuals of Leptobrachium to evaluate their morphological differences.
The morphological differences expressed by analysis of variance (ANOVA) and principal component
analysis (PCA) revealed significant differences between each Leptobrachium and four morphological
characters suitable for species identification (the iris color, color pattern, humeral and femoral gland).
The morphological differences also showed that the Bali population probably is undescribed species.


amphibia; ANOVA; identification; litter frog; morphometry; PCA

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