Hari Sutrisno
| Abstract views: 457 | PDF views: 347


Systematic of Aganaine moths has been long in dispute since they show both noctuids and arctiids morphological characteristics. Even the relationship among genera within Indonesian Aganaines is still unclear, and their phylogenetic relationships need to be reexamined since the morphological hypothesis proposed previously was not able to show the relationship among them. In order to clarify the phylogenetic relationship among five genera of Indonesian Aganaines, I used sequence of mitochondrial CO I gene (610-bp) to reconstruct their phylogenetic relationship using MP and NJ tree building methods. The results showed that the phylogenetic relationship proposed in this study contradicts the previous hypothesis. The monophyly of subfamily Aganainae has a strong bootstrap support at any tree building methods (88-95%). Neochera was divided into two clades and branched off first and then was followed by Euplocia, Peridrome, Agape, and Asota. The similarity between the previous hypothesis and this study is only on the sister-group relationship between Euplocia and Peridrome and the division of Neochera into two clades. The synapomorphy of Euplocia + Peridrome is a large androconial patch on the forewing upperside at the costal base. This study also showed that all internal nodes gained least supports. It indicates that the relationships among internal nodes proposed here were poorly supported due to the limited number of species and only a short fragment of one mitochondrial gene included in the analysis. Further studies are needed to be done by including more other species, other nuclear genes, and genitalia characters in order to test the validity of the relationships proposed here.

Key words: Aganaine, Asota, Agape, Euplocia, Peridrome, Neochera, phylogeny


Aganaine, Asota, Agape, Euplocia, Peridrome, Neochera, phylogeny

Full Text:




Caterino, M.S. & F.A. Sperling, 1999. Papilio Phylogeny Based on Mitochon-drial Cytochrome Oxidase I and II genes. Molecular Phylogeny and Evolution 11(1), 122-137.

Common, I.F.B., 1990. Moth of Australia. Melbourne University Press Carlton. 535 pp.

DeSalle, R.T., E.M. Freedman & A.C. Wilson, 1987. Tempo and mode of se-quence evolution in mitochondrial DNA of hawaiian Drosophila. Jour-nal of Molecular Evolution 26: 157-164.

Fibiger, M & J.D. Lafontaine, 2005. A review of the higher classification of the Noctuoidea (Lepidoptera) with special reference to the Holarctic fauna. Esperiana 11: 7-92.

Goto, S.G. & M.T. Kimura, 2001. Phylogenetic utility of mitochondrial CO I and nuclear Gpdh gene in Drosophila. Molecular Phylogeny and Evolution 18: 404-422.

Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for window 95/98/TNT. Nucleic Acid Symposium Series 41: 95-98.

Hasegawa, M., H. Kishino & T. Yano. 1985. Dating the human-ape splitting by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution 22: 160-174.

Hebert, P.D., J.R. Dewaard & J.F. Landry, 2010. DNA barcodes for 1/1000 of the animal kingdom. Biology Letter 6 (3): 359-362.

Holloway, J.D., 1988. The Moths of Borneo: family Arctiidae, subfamilies Syn-tominae, Euchominae, Arctiinae; Noctuidae misplaced in Arctiidae(Camptolomia, Aganainae). Malayan Nature Society, Kuala Lumpur.

Irwin, D.M., T.D. Kocher & A.C. Wilson, 1991. Evolution of the cytochrome b gene of mammals. Journal of Molecular Evolution 32: 128-144.

Kimura, M., 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. Jour-nal of Molecular Evolution 16: 111-120.

Kitching, I.J. & J.E. Rawlins, 1998. The Noctuoidea. In Kristensen, N.P. (eds). Lepidoptera Vol 1. Handbuch der Zoologie, de Gruyter, Berlin, pp. 355-402.

Lafontaine, D.J. & M. Fibiger, 2006. Revised higher classification of the Noc-tuoidea (Lepidoptera). Cannadian Entomology 138: 610-635.

Landry, B., J.A. Powell & F.A.H. Sperling, 1999. Systematics of the Argyro-taenia franciscana (Lepidoptera: Tortricidae) Species Group: Evidence from Mitochondrial DNA. Systematics 92(1): 40-46.

Mitchell, A., C. Mitter & J.C. Regier, 2006. Systematics and evolution of the cutworm moths (Lepidoptera: Noctuidae): evidence from two protein-coding nuclear genes. Systematic Entomology 31: 21-46.

Munroe, E.G., 1982. Lepidoptera. In Parker, S.P. (eds). Synopsis and Classifica-tion of Living Organisms. vol. 2. McGraw-Hill, New York, pp. 612-651.

Nei, M. & S. Kumar, 2000. Molecular Evolution and Phylogenetics. London: Oxford University Press.

Rubinoff, D. & F.A.H. Sperling, 2002. Evolution of ecological traits and wing morphology in Hemileuca (Saturniidae) based on a two-gene phylogeny. Molecular Phylogeny and Evolution 25: 70-86.

Simon, C., F. Frati, A.T. Beckenbach, B. Crespi, H. Liu & P. Flook, 1994. Evo-lution, Weighting, and Phylogenetic utility of Mitochondrial Gene Se-quences and a Compilation of conserved Polymerase Chain Reaction Primers. Annals Entomological Society 87(6): 651-701.

Sperling, F.A.H., & D.A. Hickey, 1994. Mitochondrial DNA Sequences Varia-tion in the Spruce Budworm Species Complex (Choristoneura: Lepidop-tera). Molecular Biology and Evolution 1(4): 656-665.

Sutrisno, H., N. Azuma & S. Higashi, 2006. Molecular phylogeny of the Indo-Australia Glyphodes and allied genera (Insecta: Lepidoptera: Cram-bidae) inferred from CO I, CO II and EF-1 alpha genes. Journal of Spe-cies Diversity 11: 57-69.

Sutrisno, H., 2003. Phylogeny of Glyphodes Guenee (Lepidoptera: Crambidae: Spilomelinae) based on nucleotide sequence variation in a mitochondrial CO I gene: congruence with Morphological data. Treubia 33(1): 35-42.

Sutrisno, H., 2006. Evolution of a Wingless gene and its Utility for inferring the relationships within Glyphodes Moths. Hayati (Journal of Bioscience) 13(4): 145-150.

Sutrisno, H., 2008. Species Status of yellow stem borer Scirpophaga incertulas (Lepidoptera: Pyralidae) based on CO I gene sequences. Treubia 36: 37-47.

Swofford, D.L., 2001. PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Version 4.0b10 for 32-bit Microsoft Windows. Sinauer Associates, Sunderland, Massachusetts.

Toki, W & K. Kubota, 2010. Molecular Phylogeny based on Mitochondrial genes and evolution of host plant use in the long-horned beetle Tribe Lamiini (Coleoptera: Cerambycidae) in Japan. Environmental Entomol-ogy 39 (4): 1336-1343.

Yang, Z., 2008. Computational Molecular Evolution. London: Oxford Univer-sity Press, 357 pp.

Copyright (c) 2015 Research Center for Biology, Indonesian Institute of Sciences (LIPI)


  • There are currently no refbacks.