Hubungan Filogenetik Phrynella pulchra Boulenger, 1887 Berdasarkan Gen 16S rRNA

Farid Akhsani, Amir Hamidy, Achmad Farajallah, Eric N. Smith
10.52508/zi.v26i2.3721
| Abstract views: 913 | PDF views: 2863

Abstract

Phrynella merupakan marga monotipik yang sejauh ini hanya terdiri dari satu jenis, yakni Phrynella pulchra. Jenis ini terdistribusi di Semenanjung Malaysia, Sumatra dan Pulau Mentawai. Kami mengevaluasi status taksonomi populasi yang berasal dari Sumatra menggunakan data sekuen dari gen 16S rRNA mitokondria. Hubungan filogenetik dianalisis menggunakan Neighbour Joining (NJ), Maximum Likelihood (ML), Bayesian Inference Analisys (BI) dan Unweighted Pair Group Method with Arithmetic Means (UPGMA). Hasil penelitian menunjukkan bahwa P. pulchra adalah kelompok monofiletik terhadap anggota luar (Metaphrynella, Kaloula dan Micryletta). Kelompok monofiletik P. pulchra terbagi menjadi dua kelompok besar: Sumatra dan Semenanjung Malaysia. Kelompok Sumatra terdiri dari dua subkelompok: Aceh dan Sumatra Utara- Bengkulu. Jarak genetik (uncorrected p-distance) antara populasi Semenanjung Malaysia dan Sumatra berkisar antara 1,1 sampai 2,0%, sedangkan di dalam populasi Sumatra berkisar antara 0,0 sampai 1,1%. Rendahnya jarak genetik populasi Semenanjung Malaysia dengan Sumatra secara taksonomi menunjukkan populasi tersebut masih berada pada tingkat Jenis yang sama.

Keywords

Mitokondria, populasi, jenis, Sumatra, taksonomi

Full Text:

PDF

References

Blackburn, D. C., Siler, C. D., Diesmos, A. C., McGuire, J. A., Cannatella, D. C. & Brown, R. M. (2013). An adaptive radiation of frogs in a Southeast Asian island archipelago. Evolution, 67, 2631–2646.

Boulenger, G. A. (1887). On new batrachians from Malacca. Annual Magazine National History, 5, 345–348.

Boulenger, G. A. (1890). List of the reptiles,

batrachians, and freshwater fishes col-lected by Professor Moesch and Mr. Iversen in the distrivt of Deli, Suma-tra. Proceedings of the Zoological So-ciety of London, 1890, 30–39.

de Sá, R. O., Streicher, J. W., Sekonyela, R., Forlani, M. C., Loader, S. P.,Greenbaum, E., Richards, S. J., &Haddad, C. F. B. (2012). Molecular phylogeny of microhylid frogs (Anura: Microhylidae) with emphasis on relationships among New World genera. BMC Evolutionary Biology, 12, 241, 1–21.

Frost, D. R., Grant, T., Faivovich, J., Bain, R. H., Haas, A., Haddad, C. F. B., de Sá, R. O., Channing, A., Wilkinson, M., Donnellan, S. C., Raxworthy, C. J., Campbell, J. A., Blotto, B. L., Moler, P. E., Drewes, R. C., Nussbaum, R. A., Lynch, J. D., Green, D. M. & Wheeler W. C. (2006). The amphibian tree of life. Bulletin of the AMNH, 297, 1–370.

Frost, D.R. (2016). Amphibian Species of the World: an Online Reference. Version 6.0 [online]. Diambil dari http://research.amnh.org/herpetology/amphibia/index.html. American Mu-seum of Natural History, New York, USA. [Diakses 27 November 2016].

Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N. J. (2007). Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS ONE, 2(10), e1109.

Gunther, A. (1887) Notes on Batrachians from Perak. Annual Magazine National History, 6, 312–316.

Hamidy, A., Matsui, M., Nishikawa, K., Be-labut, D. M. (2012). Detection of cryptic taxa in Leptobrachium nigrops (Amphibia, Anura, Megophryidae) with description of two new species, Zootaxa, 3398, 22–39.

Hamidy, A., Matsui, M., Shimada, T., Nishi-kawa, K., Yambun, P., Sudin, A., Kusrini, M.D. & Kurniati, H. (2011). Morphological and genetic discord-ance in two species of Bornean Lep-tobrachium (Amphibia, Anura, Megophryidae). Molecular Phyloge-netics and Evolution, 61, 904–913.

Hawkins, T. L., O’Connor-Morin, T, Roy, A. & Santillan, C. (1994) DNA purifica-tion and isolation using a solid-phase. Nucleic acids Res, 22, 4543–4544.

Hedges, S. B. (1994). Molecular evidence for the origin of birds. Proceedings of the National Academy of Science USA, 91, 2621–2624.

Hillis, D. M., Mable, B. K., Larson, A., Davis, S. K., Zimmer, E. A., 1996. Nucleic acids IV: sequencing and cloning. In: Hillis, D.M., Mable, B.K., Moritz, C. (Eds.), Molecular Systematics, second ed. Sinauer, Sunderland, pp. 321–406.

Huelsenbeck, J, P. & Hillis, D. M. (1993). Success of phylogenetic method in the four taxon case. Systematics Biology, 42, 247–264.

ICZN (1985). International Code of Zoological Nomenclature, adopted by the XV International Congress of Zoology. International Trust for Zoological Nomenclature, London. 338 pp.

Inger, R. F. & Voris, H. (2001). The biogeo-graphical relations of the fogs and snakes of Sundaland. Journal of Bio-geography 28, 863–891.

Kurabayashi, A., Matsui, M., Bellabut, D. M., Yong, H. S., Norhayati, A., Sudin, A., Kuramoto, M., Hamidy, A. & Sumida, M. (2011) From Antarctica or Asia? New colonization scenario for Aus-tralian-New Guinean narrow mouth toads suggested from the findings on a mysterious genus Gastrophrynoides. BMC Evolutionary Biology, 11, 175.

Leaché, A. D. & Reeder, T. W. (2002). Molec-ular systematics of the eastern fence lizard (Sceloporus undulatus): a com-parison of parsimony, likelihood, and Bayesian approaches. Systematics Biology, 51, 44–68.

Matsui, M., Hamidy, A., Kuraishi, N. (2014). A new Species of Polypedates from Sumatra, Indonesia (Amphibia: Anu-ra). Species Diversity, 19, 1–7.

Matsui, M., Hamidy, A., Murphy, R. M., Khonsue, W., Yambun, P., Shimada, T., Ahmad, N. Belabut, D. M., Jiang, J.P. (2010). Phylogenetic relationships of megophryid frogs of the genus Lep-tobrachium (Amphibia, Anura) as re-vealed by mtDNA gene sequences.Molecular Phylogenetics and Evolu-tion, 56, 259–272.

Matsui, M., Hamidy, A., Belabut, D. M., Ah-mad, N., Panha, S., Sudin, A., Khon-sue, W., Oh, H. S., Yong, J. P., Jiang, J. P. & Nishikawa, K. (2011). System-atic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylo-genetics and Evolution, 61, 167–179.

Parker, H. W. (1934). A monograph of a frogs of the family microhylidae. London (GB): Printed by order pf the British Museum.

Smith, M. A. (1926). Spolia Mentawia: Rep-tiles and amphibians. Annual Magazine National History, 9, 76–81.

Smith, M. A. (1930). The Reptilia and Am-phibia of the Malay Peninsula. A sup-plement to GA. Boulenger’s Reptilia and Batrachia, 1912. Bulletin of the Raffles Museum. Singapore 3: xviii + 149.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011). MEGA5: molecular evolu-tionary genetics analysis using maxi-mum likelihood, evolutionary dis-tance, and maximum parsimony methods. Molecular Biology and Evolution, 10, 2731–2739.

Tanabe, A. S., (2007). Kakusan: a computer program to automate the selection of a nucleotide substitution model and the configuration of a mixed model on multilocus data. Molecular Ecolo-gy Notes, 7, 962–964.

The IUCN Red List of Threatened Species. Version 2017-3. Diambil dari [www.iucnredlist.org] tanggal 26 December 2017.

Vences, M., Thomas, M., Meijden, A., Chiari, Y. & Vietes, D.R. (2004). Compara-tive performance of the 16S rRNA gene in DNA barcoding of amphibi-ans. Frontiers in Zoology, 2(5), 1–12.

van der Meijden, A., Vences, M., Hoegg, S., Boistel, R., Channing, A. & Meyer, A. (2007). Nuclear gene phylogeny of narrow-mouthed toads (Family Micro-hylidae) and a discussion of compet-ing hypotheses concerning their bio-geo- graphical origins. Moleculer Phylogenetic Evolution, 44, 1017–1030.

DOI: https://doi.org/10.52508/zi.v26i2.3721

Refbacks

  • There are currently no refbacks.