MORFOLOGI DAN MORFOMETRI SPERMATOZOA MUSANG LUWAK (Paradoxurus hermaphroditus)

Ragil Angga Prastiya, Diana Santi, Ade Miftakhul Aziz, Hanun Putri Nuraida
| Abstract views: 1406 | PDF (Bahasa Indonesia) views: 1501

Abstract

Analisis morfologi dan morfometri spermatozoa musang dengan metode pewarnaan khusus Eosin-Nigrosin dapat digunakan untuk mengukur kualitas kesehatan reproduksi musang jantan melalui hasil pengamatan abnormalitas morfologi spermatozoa dan nilai morfometri, serta dapat membantu dalam upaya peningkatan populasi musang. Musang (Paradoxurus hermaphroditus) adalah mamalia liar yang diklasifikasikan ke dalam keluarga Viverridae dan termasuk dalam genus Paradoxurus. Musang dikategorikan  least concern berdasar International Union for Conservation of Nature (IUCN). Salah satu cara untuk melestarikan musang adalah dengan mempelajari struktur dan fungsi dari organ reproduksi musang jantan, salah satunya melalui pengamatan morfologi dan  morfometri spermatozoa. Penelitian ini menggunakan 8 ekor musang jantan yang berusia 1-3 tahun dan dewasa kelamin. Sebanyak 100 sampel spermatozoa diamati kemudian diukur panjang kepala, lebar kepala, perimeter atau lingkar kepala, luas area, ellipticity, elongation, roughness dan regularity nya. Parameter morfologi dijelaskan secara deskriptif dengan melihat morfologi normal dan abnormal dari spermatozoa musang. Hasil penelitian ini menunjukkan bahwa spermatozoa musang memiliki panjang kepala 9,67±0,60 μm, lebar kepala 4,73±0,37 μm, luas area 40,51± 4,60 μm2, perimeter atau lingkar spermatozoa 22,64±1,24 μm, ellipticity 2.05±0,017 μm, elongation 0.34±0.04 μm, roughness 0.99±0.07 μm, regularity 0.89±0.07 μm, panjang ekor 62.01±1.76 μm, dan panjang total spermatozoa 71,68±1,89 μm. Dengan demikian dapat disimpulkan bahwa pengamatan morfometri spermatozoa pada musang dapat digunakan sebagai salah satu parameter uji fertilitas musang jantan. 

 

Keywords

Musang, morfologi, morfometri, spermatozoa, Eosin-Nigrosin

References

Arifiantini, R. I., Wresdiyati, T., & Retnani, E. F. (2006). Kaji Banding Morfometri Spermatozoa Sapi Bali (Bos sondaicus) Menggunakan Pewarnaan Williams, Eosin, Eosin Nigrosin dan Formol-Saline. Jurnal Sains Veteriner, 24(1), 65-70.

Aristides, Y., Agus, P., & Adji, S. (2016). Perlindungan Satwa Langka di Indonesia Dari Perspektif Convention On International Trade In Endangered Species Of Flora And Fauna (CITES). Diponegoro Law Journal, 5(4), 1-17.

Axner, E. & Linde-Forsberg, C. (2002). Semen Collection and Assessment, and Artificial Insemination in the Cat. International Veterinary Information Service.132.

Baker, N. & Kelvin, L. (2008). Wild Animals of Singapore: A Photographic Guide to Mammals, Reptiles, Amphibians, and Freshwater Fishes. Singapura: Vertebrate Study Group.

Banaszewska, D., Andraszek, K., Czubaszek, M., & Biesiada–Drzazga, B. (2015). The Effect of Selected Staining Techniques on Bull Sperm Morphometry. Animal reproduction science, 159, 17-24.

Bjorndahl, L., Soderlund, I., & Kvist, U. (2003). Evaluation of the One Step Eosin-Nigrosin Staining Technique for Human Sperm Vitality Assessment. Human reproduction, 18(4), 813-816.

Budianto, I. L., Waluyanto, H. D., & Zacky, A. (2020). Perancangan Artbook Untuk Meningkatkan Awareness Masyarakat Terhadap Kelangkaan Fauna Indonesia. Jurnal DKV Adiwarna, 16, 1-11

Chenoweth, P. J. (2005). Genetic Sperm Defect. Theriogenology, 64, 457-468.

Czubaszek, M., Andraszek, K., Banaszewska, D., & Walczak-Jędrzejowska, R. (2019). The Effect of the Staining Technique on Morphological and Morphometric Parameters of Boar Sperm. Plos One, 14(3), 1-17

Fitzpatrick, J., Montgomerie, R., Desjardins, J. K., Stiver, K. A., Kolm, N., & Balshine, S. (2009). Female promiscuity promotes the evolution of faster sperm in cichlid fishes. Proceedings of the National Academy of Sciences of the United States of America, 106, 1128–1132.

Gage, M. J. G. & Freckleton, R. P. (2003). Relative Testis Size and Sperm Morphometry Across Mammals: No Evidence For An Association Between Sperm Competition and Sperm Length. Proceedings of the Royal Society B., 270, 625-632.

Gizejewski, Z., Marta, W.,& Jolanta, P.(2002). Seasonal Changes in the Dimensions of Red Deer (Cervus elaphus) Spermatozoa. M. Polish Academy of Sciences. Research Station for Ecologycal Agriculture and Preserve Animal Breeding. Poland.

Gontarz, A., Banaszewska, D., Gryzińska, M., & Andraszek, K. (2016). Differences in Drone Sperm Morphometry and Activity at the Beginning and End of the Season. Turkish Journal of Veterinary & Animal Sciences, 40, 1511-1526.

Ihsan, N. M. (2009). Bioteknologi Reproduksi Ternak. Universitas Brawijaya. Malang.

Kaiin, E. M., Gunawan, M., & Maulana, T. (2017). Morphometry and Abnormality Evaluation of Sex-Sorted Sperm of Spotted Buffalo (Tedong bonga). Nusantara Bioscience, 9(2), 175-180.

Kondracki, S., Banaszewska, D., & Mielnicka, C. (2005). The Effect of Age on the Morphometric Sperm Traits of Domestic Pigs (Sus scrofa domestica). Cellular & Molecular Biology Letters, 10(1), 3-13.

Maree, L., Du Plessis, S. S., Menkveld, R., & Van der Horst,G. (2010). Morphometric Dimensions of the Human Sperm Head Depend on the Staining Method Used. Human Reproduction, 25(6), 1369-1382.

McGowan, M., Galloway,D., Taylor, Entwistle, P., & Johnston, P. (1995). Veterinarians Examination of Bulls. Queensland: Australia Association of Cattle Veterinarians.

Morrell, J. M. & Martinez, H. R. (2009). Biomimetic Techniques For Im-proving Sperm Quality in Animal Breeding: A Review. The Open Andrology Journal,1, 1-9.

Natalia, F., Sardjito, T., Adikara, R. T. S., Utama, S., Warsito, S. H., & Srianto, P. (2016). Kajian Morfometri Spermatozoa Terejakulasi Sugar Glider (Petaurus breviceps papuanus). Ovozoa, 5(1), 9-12.

Novelina, S., Shandy, M. P., Chairun, N., & Heru, S. (2014). Tinjauan Makroskopik Organ Reproduksi Jantan Musang Luwak (Paradoxurus hermaphroditus). Acta Veterinaria Indonesiana, 2(1), 26-30.

Patmawan, E. F., Gaina, C. D., & Foeh, N. D. (2020). Morfologi Abnormalitas Spermatozoa Babi Landrace Dan Babi Duroc dengan Pewarnaan Carbofuchsin. Jurnal Veteriner Nusantara, 3(2), 113-119.

Pena, F. J., Saravia, F., Garcia, H. M., Nunez, M. I., Tapia, J. A., & Johannisson, A. (2005). Identification of Sperm Morphometric Subpopulations in Two Different Portions of the Boar Ejaculate and Its Relation to Post Thaw QuaIity. Journal of Andrology, 26, 716-723.

Prastiya, R. A., Prastika, Z., & Andriyani, A. (2021). Quality and morphometric char-acters of spermatozoa in two native bull (Pesisir and Rambon) in Indonesia. AIP Publishing LLC Conference Proceedings, 2353(1), 030029.

Prastiya, R. A., Rimayanti, Munir, M. M., & Nugroho, A. P. (2021). The Protective Impacts of α-tocopherol Supplementation on the Semen Quality of Sapera Goat Preserved at 4⁰C. Tropical Animal Science Journal, 44(3), 261-266.

Putri, R. D. A., Gunawan, M., & Kaiin, E. M. (2002). Seasonal Changes in the Dimensions of Red Deer (Cervus elaphus) Spermatozoa. M. Polish Academy of Sciences. Research Station for Ecologycal Agriculture and Preserve Animal Breeding. Poland.

Rahmawati, E. (2007). Pemanfaatan Teknologi Informasi (TI) untuk pengukuran Luas Permukaan Kepala Sel Spermatozoa Domba. Surabaya: Fakultas Kedokteran Hewan Universitas Airlangga.

Rijsselaere, T., Van Soom, A., Hoflack, G., Meas, D., & De Kruif, A. (2004). Automated Sperm Morphometry and Morphology Analysis of Canine by the Hamilton-Thorne Analyser. Theriogenology, 62, 1292-1306.

Roy, S. C. & Atreja, S. K. (2008). Effect of Reactive Oxygen Species on Capacitation and Associated Protein Tyrosine Phosphorylation in Buffalo (Bubalus bubalis) Spermatozoa. Animal reproduction science, 107(1-2), 68-84.

Saputro, A. L., Hermadi, H. A., & Sosiawati, S. M. (2016). Kualitas Spermatozoa Domba Merino pada Sisi Anoda Hasil Pemisahan dengan Teknik ESS (Electric Separating Sperm). Veterinaria Medika, 9(3), 65.

Schipper, J., Hoffmann, M., Duckworth, J. W., & Conroy, J. (2008). The 2008 IUCN Red Listings of The World’s Small Carnivores. Small Carnivore Conservation, 39, 29-34.

Solihati, N. (2017). Evaluation The Natural Proportion of XY Chromosome Bearing Sperm of West Java Local Ram Using Morfometric Methode. International Seminar on Tropical Animal Production (ISTAP), 782-787.

Supriatna, S. & Mimin, A. (2014). Analisis Strategi Pengembangan Usaha Kopi Luwak (Studi Kasus UMKM Careuh Coffee Rancabali-Ciwidey, Bandung). Jurnal Manajemen dan Organisasi, 5(2), 227-243.

Tasdemir, I.,Tasdemir, M., Tavukcuoglu, S., Kahraman, S., & Biberoglu, K. (2002). Effect of Abnormal Sperm Head Morphology on the Outcome of Intracytoplasmic Sperm Injection in Humans. Human Reproduction, 12, 1214-1217.

Tethool, A. N. & Purwaningsih. (2019). Efek Pemberian Ekstrak Kayu Akway (Drymis sp.) terhadap Kualitas Spermatozoa Mencit (Mus musculus L). Jurnal Ilmu Peternakan Dan Veteriner Tropis, 9(1), 24-31.

Thurston, L. M., Watson, P. F., Mileham, A. J., & Holt, W. V. (2001). Morphologically Distinct Sperm Subpopulations Defined by Fourier Shape Descriptors in Fresh Ejaculates Correlate with Variation in Boar Semen Quality Following Cryo-preservation. Journal of Andrology, 22, 382–394.

Tourmente, M., Gomendio, M., & Roldan, E. R. S. (2011). Sperm Competition and the Evolution of Sperm Design in Mammals. BMC Evolutionary Biology, 11(12), 1-10.

Vaughan, T. A.,Ryan, J. M., & Czaplewski, N. J. (2000). Mammalogy. 4th Ed. Journal of Mammalogy, 81, 916-920.

Wurlina, W., Hariadi, M., Susilowati, S., & Meles, D. K. (2020). The effect of crude guava leaf tannins on motility, viability, and intact plasma membrane of stored spermatozoa of Etawa crossbred goats. Veterinary world, 13(3), 530-535.


Refbacks

  • There are currently no refbacks.